X-Ray Datura Experiments

I exposed some Datura pollen to x-rays and pollinated some flowers as soon as they opened with the pollen. I just collected pollen using a pollinating brush set the head into the mutagenesis chamber's germplasm ring and turned it on. I set the voltage to 3vdc (which runs into a HV taser type unit) and let it run for 5 minutes (9/19/2019). I used a cut up boba straw to create rings to mark the ~8 flowers I pollinated with the x-ray pollen.

I'm trying to come up with a way to easily test the amount of poisonous alkaloids and see if I can find a mutant with less/no dangerous alkaloids.

Here are some pictures:

10-6-19 DaturaPod 10-6-19 NoPod 11-11-19 SeedBag 11-19-19 SeedCollection 11-20-19 SeedPlate
  1. About 3 pods formed (or formed that retained their markers, I found that some markers had fallen off)
  2. A couple pods didn't form, but retained their tag
  3. I put bags around the pods in order to collect the seeds, I'd use a cheese cloth or something instead.
  4. Here's the seed exactly 2 months after pollination about to be spread out and dried
  5. Seeds all dried


Planted the seeds in "Planter's Pride" Pellets made with compressed coconut coir. They expand when you soak them in water, I planted 10 with seeds I'm sure were from x-ray pollen flowers, and then the last 5 were questionable ones. I have then under the growlight where I grew the fast plants.


Just noticed this one germinated:


I was about to mix up some gibberellic acid solution too!


Another germinated today, after a blast or two of 50ppm GA3 a couple days ago. This was one of the late opening pod seeds. I need to figure out a way to mark them, I have some 1x1 inch labels I can laser print easily, maybe something with that and a toothpick?


The 4th seed germinated today, I've just used a sharpie to label them "A" through "D" so far. X is for x-ray and ? is the questionable pod.

Here's a list of germination dates (I will update as more sprout):

  • A 2/2/20 (X)
  • B 2/9/20 (?)
  • C 2/12/20 (?)
  • D 2/23/20 (?)

Tags: xray