X-Ray Datura Experiments

I exposed some Datura pollen to x-rays and pollinated some flowers as soon as they opened with the pollen. I just collected pollen using a pollinating brush set the head into the mutagenesis chamber's germplasm ring and turned it on. I set the voltage to 3vdc (which runs into a HV taser type unit) and let it run for 5 minutes (9/19/2019). I used a cut up boba straw to create rings to mark the ~8 flowers I pollinated with the x-ray pollen.

I'm trying to come up with a way to easily test the amount of poisonous alkaloids and see if I can find a mutant with less/no dangerous alkaloids.

Here are some pictures:

10-6-19 DaturaPod 10-6-19 NoPod 11-11-19 SeedBag 11-19-19 SeedCollection 11-20-19 SeedPlate
  1. About 3 pods formed (or formed that retained their markers, I found that some markers had fallen off)
  2. A couple pods didn't form, but retained their tag
  3. I put bags around the pods in order to collect the seeds, I'd use a cheese cloth or something instead.
  4. Here's the seed exactly 2 months after pollination about to be spread out and dried
  5. Seeds all dried

1/11/2020

Planted the seeds in "Planter's Pride" Pellets made with compressed coconut coir. They expand when you soak them in water, I planted 10 with seeds I'm sure were from x-ray pollen flowers, and then the last 5 were questionable ones. I have then under the growlight where I grew the fast plants.


2/02/2020

Just noticed this one germinated:

datura-seedling

I was about to mix up some gibberellic acid solution too!


2/08/2020

Another germinated today, after a blast or two of 50ppm GA3 a couple days ago. This was one of the late opening pod seeds. I need to figure out a way to mark them, I have some 1x1 inch labels I can laser print easily, maybe something with that and a toothpick?


2/24/2020

The 4th seed germinated today, I've just used a sharpie to label them "A" through "D" so far. X is for x-ray and ? is the questionable pod.

Here's a list of germination dates (I will update as more sprout):

  • A 2/2/20 (X)
  • B 2/9/20 (?)
  • C 2/12/20 (?)
  • D 2/23/20 (?)

Tags: xray